DNA
ChiP

Part:BBa_K564004:Design

Designed by: Anna Lewinska   Group: iGEM11_DTU-Denmark   (2011-09-19)

ChiP promoter and 5' UTR; WT RBS (AAGAGG)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no design consideration


Source

It was obtained by PCR from Escherichia Coli W3110 genomic DNA.

References

Caught at its own game:regulatory small RNA inactivated by an inducible transcript mimicking its target. Figueroa-Bossi et al. 2009, Genes and development 23:2004-2015 Switching off small RNA regulation with trap-mRNA Overgaard et al. 2009, Molecular Microbiology 73(5):790-800